Paper Strip-Based Sensor for Detection of Antimicrobial Resistant Listeria monocytogenes in Milk

Clicks: 9
ID: 337808
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #232 of 241 articles by views in FEMS microbiology letters

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 241 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract To develop a rapid, selective, and field-deployable paper strip assay for the detection of Listeria monocytogenes and targeted screening of resistance to Penicillin G and Ampicillin in milk. A novel Listeria Selective Enrichment Medium (LSEM) was optimized to enhance the recovery of L. monocytogenes in milk while reducing interference from selected competing microorganisms evaluated in this study. The assay employed β-D-glucosidase and enzyme-substrate reactions for presumptive and species-specific detection, producing characteristic colour changes for Listeria spp. The developed paper strip detected L. monocytogenes within 7 h in broth and 9 h (with enrichment for 24 h at 1 CFU/25 mL) in spiked sterilized milk. The total detection time of the developed assay was approximately 33 h (24 h enrichment + 9 h strip-based confirmation). In the present study, antimicrobial resistance assessment was limited to two representative β-lactam antibiotics, Penicillin G and Ampicillin. Therefore, the assay should currently be considered a targeted screening platform rather than a universal antimicrobial susceptibility test. Resistant strains showed colour development in the presence of the respective antibiotic, whereas susceptible strains remained colourless. Validation with 70 milk samples confirmed one raw milk sample positive for L. monocytogenes, which was further verified using ISO 11290–1:2017 and disc diffusion methods. The developed assay provides a rapid, selective, cost-effective, and user-friendly platform for simultaneous detection of L. monocytogenes and targeted susceptibility screening to Penicillin G and Ampicillin in milk.
Reference Key
openalex_W7224566100 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Shreya Talan, Mandeep Balhara, Shabnam Kouser, Naresh Kumar, Raghu Hirikyathanahalli Vishweswaraiah
Are you one of these authors? Claim your profile
Journal FEMS microbiology letters
Year 2026
DOI
10.1093/femsle/fnag132
URL
https://doi.org/10.1093/femsle/fnag132
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.