Condensins-mediated mitotic folding persists as a structural echo in interphase chromatin

Clicks: 15
ID: 333785
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #1,217 of 1,296 articles by views in Nucleic Acids Research

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 1,296 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract The structural maintenance of chromosomes (SMC) protein complexes, cohesin and condensins, orchestrate cell cycle-dependent transitions in chromosome folding: from the relatively decondensed interphase chromatin, organized into dynamic DNA loops by cohesin, to the highly condensed arrays of DNA loops in mitotic chromosomes formed by condensins. Here, using Hi-C data from mouse embryonic stem cell lines with auxin-inducible degron-tagged subunits of SMC complexes, we clarify the role of condensins in shaping chromatin contacts within the interphase nucleus. We found that depletion of condensin I leads to weakened segregation of chromatin into A and B compartments. We also show that the contact patterns established by condensins during mitosis are preserved in interphase, suggesting a structural memory of mitotic chromosome folding. Condensins influence chromocenter organization: in the absence of condensin II during mitosis, centromeres tend to cluster, forming hyperclusters of pericentric heterochromatin. In contrast, depletion of condensin I reduces the number of heterochromatin clusters, consistent with enhanced chromosome territoriality. By combining a cohesin-degron system with Mcph1 knockout, we established an artificial model in which condensin II becomes the major driver of chromatin folding in interphase nuclei following cohesin depletion. Unlike extrusive cohesin, condensin II does not stall at sequence-specific sites and forms large, randomly positioned loops on interphase chromatin. Moreover, condensin II activity during interphase does not interfere with cohesin-dependent structures such as TADs and chromatin loops.
Reference Key
openalex_W7223469099 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Anastasia Yunusova, Egor Tsoi, A. V. Smirnov, Т. А. Шнайдер, Inna Pristyazhnuk, Maria M. Gridina, Ф Казаков Евгений, Igor I. Kireev, Ivan Myakinkov, Miroslav Nuriddinov, Veniamin Fishman, Нариман Рашитович Баттулин
Are you one of these authors? Claim your profile
Journal Nucleic Acids Research
Year 2026
DOI
10.1093/nar/gkag1014
URL
https://doi.org/10.1093/nar/gkag1014
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.