Development of a Simple and Rapid HPLC-UV Method for Simultaneous Determination of Ciprofloxacin Hydrochloride and Oxytetracycline Hydrochloride in Aqueous Matrix

Clicks: 2
ID: 333377
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #372 of 374 articles by views in journal of aoac international

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 374 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract Background Reliable and effective methods capable of simultaneously determining ciprofloxacin hydrochloride (CIP) and oxytetracycline hydrochloride (OTC) can be useful when concentrations fall within the validated working range. Objective This study aimed to develop and validate a simple and rapid reversed-phase high-performance liquid chromatography with ultraviolet detection (RP-HPLC-UV) method for simultaneous determination of CIP and OTC in aqueous matrices. Methods The separation was performed using an Agilent InfinityLab Poroshell 120 EC-C18 column (150 × 4.6 mm, 4 μm) with a mobile phase consisting of 0.1% formic acid and acetonitrile at a flow rate of 1.0 mL/min. The method was validated for linearity, precision, selectivity, recovery, sensitivity, robustness and system suitability. Matrix performance was assessed using fortified lake-water samples. Results Linearity over the concentration range of 10–25 mg/L, with R² values of 0.9947 for OTC and 0.9923 for CIP was achieved. The LOD values were 1.40 mg/L for OTC and 1.69 mg/L for CIP, while the corresponding LOQ values were 4.24 and 5.11 mg/L, respectively. Peak-area RSDs were below 1% in both intraday and interday precision studies. Recoveries in fortified lake water ranged from 91.12–109.13% for OTC and 82.88–107.72% for CIP and from 95.84–104.91% and 89.56–112.88%, respectively, in interday analysis. Robustness testing showed that satisfactory chromatographic separation was maintained under the investigated variations in flow rate, column temperature, and detection wavelength. Conclusion The HPLC-UV approach was applicable for the simultaneous determination of CIP and OTC in aqueous samples containing relatively elevated concentrations of the target antibiotics. The method is not intended for direct trace-level determination in typical environmental waters without an appropriate preconcentration step. Highlights A simple RP-HPLC-UV method was developed for the simultaneous determination of CIP and OTC. The method is applicable to aqueous samples containing relatively elevated antibiotic concentrations.
Reference Key
openalex_W7223152772 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Wan Chi Soon, Ahmad Zuhairi Bin Abdullah, Azam Taufik Mohd Din
Journal journal of aoac international
Year 2026
DOI
10.1093/jaoacint/qsag096
URL
https://doi.org/10.1093/jaoacint/qsag096
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.