Photosynthesis-derived carbon gates cell cycle activation to enable hormone-autonomous shoot regeneration

Clicks: 1
ID: 333340
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal

Ranked #367 of 367 articles by views in The Plant cell

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 367 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract Shoot regeneration is a powerful model for cell fate reprogramming but how it occurs in nature remains poorly understood because studies in Arabidopsis thaliana conventionally rely on in vitro assays supplemented with exogenous hormones and sugars. Here, we established the Hormone-autonomY Direct Regeneration Assay (HYDRA) in which removal of the shoot apical meristem (SAM) initiates shoot regeneration from the cotyledon-hypocotyl boundary without hormone or sugar supplementation. We identify photosynthesis-derived carbon and the boundary domain as two separable but convergent requirements for shoot regeneration in HYDRA. Following SAM removal, carbon availability increases in the boundary domain where it activates cell-cycle progression via the RETINOBLASTOMA-RELATED1 (RBR1) pathway. Carbon deprivation blocks regeneration despite induction of shoot meristem genes, indicating proliferative activation is limiting. Pre-existing shoot-competent boundary cells give rise to regenerated shoots upon cell-cycle activation, while their perturbation impairs regeneration despite sufficient carbon. Carbon therefore gates the proliferative activation of these cells, enabling functional shoot meristem formation. Additionally, exogenous carbon supply overcomes the requirement for SAM removal to induce shoot formation, indicating that carbon availability also acts as an initiation cue. Together, this study reveals an inherent capacity for hormone-autonomous shoot regeneration and identifies photosynthesis-derived carbon as a central regulator of this process.
Reference Key
openalex_W7223188596 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Yetkin Çaka Ince, Arika Takebayashi, Akira Iwase, Johanna Krahmer, Aurore Chételat, Mitsuhiro Aida, Lieven De Veylder, Keiko Sugimoto
Journal The Plant cell
Year 2026
DOI
10.1093/plcell/koag318
URL
https://doi.org/10.1093/plcell/koag318
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.