The LdWRKY23-LdFUS3 module positively regulates embryo development in lily

Clicks: 11
ID: 332475
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #364 of 383 articles by views in Journal of experimental botany

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 383 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract Post-fertilization embryo abortion is a major barrier to distant hybridization in lily, but the molecular mechanisms underlying hybrid embryo viability remain unclear. In this study, we examined embryo development in the cross between Asiatic hybrids ‘Feixiang Tiancheng’ (♀) and Lilium davidii var. unicolor (♂), and found that embryo abortion occurred at 40 days after pollination. Comparative transcriptome analysis of embryos differing in developmental stage and fate identified LdFUS3, encoding a nuclear B3-domain transcriptional activator, as a candidate regulator preferentially expressed in normal embryos. Silencing LdFUS3 or its homolog LhFUS3 in two hybrid backgrounds impaired ovary growth, increased embryo abortion, and reduced the expression of embryo-development genes, including ABI3, LEC2, and 12S, indicating a conserved positive role of FUS3 in lily hybrid embryo development. Yeast one-hybrid screening of the LdFUS3 promoter identified LdWRKY23 as an upstream regulator. LdWRKY23 localized to the nucleus, displayed transcriptional activation activity, and directly bound to and activated the LdFUS3 promoter in yeast one-hybrid, electrophoretic mobility shift assay, and dual-luciferase assays. These findings reveal that LdFUS3 as a key regulator of lily embryo development and establish the LdWRKY23-LdFUS3 transcriptional module as a positive regulatory pathway controlling hybrid embryo viability. This study provides new insight into the molecular basis of post-fertilization embryo abortion in lily and offers potential molecular targets for improving distant hybridization and lily breeding efficiency.
Reference Key
openalex_W7222045454 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Sujuan Xu, Hanhan Feng, Rui Liang, Fengfeng Cao, Ting Zhang, Ze Wu, Nianjun Teng
Journal Journal of experimental botany
Year 2026
DOI
10.1093/jxb/erag525
URL
https://doi.org/10.1093/jxb/erag525
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.