Temperature-dependent xyloglucan physicochemical changes are associated with XTH-mediated reserve mobilization in Hymenaea courbaril

Clicks: 20
ID: 332192
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #132 of 269 articles by views in Annals of botany

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 269 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract Background and Aims Development of Hymenaea courbaril seedlings requires mobilization of xyloglucan (XyG), mediated by four enzymes whose coordinated action reduces cotyledon biomass. Xyloglucan endotransglycosylase/hydrolase (XTH) can display both xyloglucan endotransglucosylase (XET) and xyloglucan endohydrolase (XEH) activities. In Hymenaea-XTH activity is oligosaccharide-dependent, suggesting XET-like behavior. XyGs react with iodine to form a blue-colored, temperature-sensitive complex. Here, this interaction was used to study the biochemical and biophysical properties of XyGs and the Hymenaea-XTH interaction in vitro and in planta across a range of temperatures. Methods We reacted XyGs from different sources with iodine and analyzed the resulting complexes across temperature cycles. Hymenaea-XTH was partially purified and assayed with various XyGs. We measured XTH transglycosylation and hydrolysis of XyG oligosaccharides at different temperatures. In planta, Hymenaea seedlings were subjected to four temperature regimes to assess responses in cotyledon reserve depletion and seedling growth. Key Results XyGs from different sources exhibit varying physicochemical properties at physiological temperatures. Hymenaea-XTH showed maximal XyG-degrading activity near 35 °C, coinciding with the temperature transition associated with changes in XyG interactions, but only when oligosaccharides were added. Modeled potential XEH activity (XDA), estimated from enzyme-polysaccharide properties and leaf surface temperature, suggested that temperature modulates the Hymenaea-XTH and XyG interaction during seedling development. A constant 35 °C accelerated cotyledon reserve depletion, whereas alternating 20/35 °C regimes maintained coupling between reserve mobilization and biomass accumulation. Conclusions Temperature-dependent changes in the XyG–iodine interaction were linked to maximal XyG-degrading activity in the Hymenaea-XTH-enriched preparation at 35 °C. These results support a proposed model in which daily warming may favor XyG modification by increasing putative polymer accessibility and enzyme activity. Under temperature treatments, a constant 35 °C accelerated cotyledon reserve depletion, whereas alternating 20/35 °C regimes better preserved the coupling between reserve depletion and biomass accumulation.
Reference Key
openalex_W7221609185 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Adriana Grandis, Miguel Jose Minhoto, Paulo H.N. de Alcântara, Fernanda Mendes de Rezende, Caio Fernandes Ferreira, Marco Aurélio Silva Tiné, Henrique Pessoa Santos, Marcos Silveira Buckeridge
Are you one of these authors? Claim your profile
Journal Annals of botany
Year 2026
DOI
10.1093/aob/mcag329
URL
https://doi.org/10.1093/aob/mcag329
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.