Genome editing of the rutin-degrading enzyme gene FtRDE2 reduces seed bitterness in Tartary buckwheat

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ID: 332058
2026
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Abstract
Abstract Tartary buckwheat (F. tataricum) is a climate-resilient pseudocereal, yet its global adoption is constrained by its bitter seeds. Here, we employed the CRISPR/Cas9 system to disrupt the rutin-degrading enzyme 2 gene (FtRDE2), which catalyses the hydrolysis of rutin to quercetin, a major contributor to bitterness. Two independent ftrde2 mutant lines showed no detectable rutinosidase activity: mutant seed extracts maintained stable rutin levels and lacked the rapid accumulation of quercetin observed in wild-type controls. Consistent with these biochemical changes, sensory evaluation showed that wild-type flour was consistently identified as more bitter than flour from either ftrde2 mutant line. These results provide direct genetic evidence that FtRDE2 is a major determinant of rutinosidase-dependent seed bitterness and demonstrate the potential of genome editing to reduce bitterness in Tartary buckwheat.
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Authors Artur Piński, Magdalena Zaranek, Joanna Lusinska, Przemysław Kopeć, Agnieszka Płażek, Paulina Pająk, Przemysław Petryszak, Anna Kostecka‐Gugała, Meiliang Zhou, Alexander Betekhtin
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Journal Journal of experimental botany
Year 2026
DOI
10.1093/jxb/erag506
URL
https://doi.org/10.1093/jxb/erag506
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