181. The Effects of in vitro LPS Stimulation on the PBMC Transcriptome of Beef Steers with Negative or Positive Residual Average Daily Gain.

Clicks: 11
ID: 330388
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #723 of 847 articles by views in italian journal of animal science

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 847 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract This study characterized the peripheral blood mononuclear cell (PBMC) transcriptomic responses to in vitro lipopolysaccharide (LPS) stimulation in beef steers with divergent residual average daily gain (RADG), a residual-based index of feed efficiency. Positive-RADG (n = 6; RADG = +0.53 kg/d) and negative-RADG (n = 6; RADG = −0.46 kg/d) crossbred steers were selected from a cohort of 41 steers (average body weight = 384 ± 5.8 kg) following a 50-d performance testing period on a high-forage total mixed ration. PBMCs were isolated from positive-RADG and negative-RADG steers and assigned to LPS stimulation (20 μg/mL for 2 hours) or untreated control. RNA was extracted, sequenced and differentially expressed genes (DEGs; FDR ≤ 0.01, |log₂FC| ≥ 1) were identified by comparison with unstimulated controls. Gene ontology (GO) enrichment analysis was performed to characterize associated biological processes (P < 0.05). Prior to LPS stimulation, one DEG was identified between RADG groups, indicating near-equivalent baseline PBMC transcriptomes. Following stimulation, 2,739 DEGs were identified in positive-RADG steers (1,484 upregulated; 1,255 downregulated), and 3,299 DEGs in negative-RADG steers (1,555 upregulated; 1,744 downregulated). GO enrichment analysis revealed activation of canonical innate immune pathways in both groups, including cytokine production, positive regulation of IL-1 and IL-1β production, and immune response. However, positive-RADG steers exhibited a broader cytokine network enrichment profile, with the highest enrichment score for positive regulation of cytokine production, encompassing genes involved in effector activation, leukocyte trafficking, and inflammatory resolution, including NOS2, STAT3, PTX3, TGFB1, and SOD1. In contrast, negative-RADG steers exhibited disproportionate enrichment of IL-1β- and IL-6-centered inflammatory pathways, with SAA3 as the most highly upregulated DEG (log₂FC = 9.18 vs. 6.80 in positive-RADG steers), consistent with a more pronounced acute-phase transcriptional response. These findings demonstrate that while RADG-divergent steers share conserved LPS-responsive signaling pathways, they differ in the architecture of their immune transcriptional responses. The broader, more integrated cytokine network activation in positive-RADG steers, compared with the concentrated IL-1β/IL-6-driven inflammatory signature in negative-RADG steers, suggests that superior feed efficiency is associated with a more coordinated immunoregulatory capacity that may reduce the energetic cost of innate immune activation and facilitate return to metabolic homeostasis.
Reference Key
openalex_W7214745682 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Yarahy Leal, Samanthia Johnson, Modoluwamu D. Idowu, Godstime Taiwo, Taylor S Sidney, Ibukun Michael Ogunade
Are you one of these authors? Claim your profile
Journal italian journal of animal science
Year 2026
DOI
10.1093/jas/skag272.272
URL
https://doi.org/10.1093/jas/skag272.272
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.