Mitigating the deleterious impact of saliva on cell-free expression sensors for point-of-use diagnostics

Clicks: 24
ID: 329905
2026
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Abstract
Abstract Point-of-use sensors that detect biomarkers in noninvasive biofluids, such as saliva, could provide individuals with valuable insights into their health and performance status at home or in the field. Cell-free biosensors have the potential to meet these requirements, as these sensors can detect a wide variety of analytes and can be stored at room temperature after freeze-drying. However, saliva inhibits the function of cell-free biosensors, in part due to the presence of interfering proteins and nucleases. Here we show simple strategies to mitigate the inhibitory effects of saliva on the function of cell-free expression systems, including the use of nuclease inhibitors and pretreatment of saliva with centrifugal filtration or heat. We also show successful implementation of our mitigation strategies to detect cortisol spiked into pooled human saliva using a cell-free biosensor. Combined, our results show that simple approaches can be used to enable the function of cell-free biosensors in saliva.
Reference Key
openalex_W7214464228 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kathryn Beabout, Svetlana Harbaugh, Jorge L. Chávez
Journal acs synthetic biology
Year 2026
DOI
10.1093/synbio/ysag011
URL
https://doi.org/10.1093/synbio/ysag011
Keywords Keywords not found

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