Ancient Nuclear Genome Duplication Events in Vertebrates Facilitated the Functional Diversification of Ohnologous Cytochrome c : Insights from Buergeria Frogs

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ID: 329747
2026
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Abstract
In vertebrates, a nuclear gene encoding mitochondrial cytochrome c (CYC) was duplicated and diverged into one with ubiquitous expression (somatic-type) and the other with testis-preferred expression (testis-type). In this study, synteny based phylogenetic analyses suggest that cyc ohnologs originated through whole genome duplication (WGD) events in the common ancestor of vertebrates and have been conserved for approximately 450-540 million years. Molecular evolutionary analyses revealed pervasive purifying selection and an approximate Phe/Tyr dichotomy at position 46 between somatic- and testis-type CYC proteins. This was the case in two closely related amphibians, Buergeria buergeri and its hot-spring-dwelling species, Buergeria japonica. The recombinant testis-type CYC proteins from B. buergeri (Bb-CYCT) and B. japonica (Bj-CYCT) exhibited significantly higher resistance to hydrogen peroxide (H2O2) than somatic-type counterpart proteins (Bb-CYCS and Bj-CYCS). Structural analysis showed that the Tyr-46 residues in the testis-type CYC proteins face the heme, suggesting a protective role against H2O2 through an oxidation of its hydroxyl group compared to the spatially corresponding Phe-46 residue in the somatic-type counterparts. When the Tyr-46 residue in Bj-CYCT was replaced with the Phe residue, the H2O2 resistance was significantly reduced, confirming the functional importance of Tyr-46. These results demonstrate that functional divergence between somatic- and testis-type Buergeria CYC proteins is at least partly attributable to the Phe-to-Tyr substitution, suggesting that ancient nuclear genome duplication contributed to tissue-specific divergence of mitochondrial function.
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openalex_W7214316333 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Yuka Asaeda, Sotaro Fujii, Hajime Ogino, Michael A. Hough, Takeshi Igawa, Yoshihiro Sambongi
Journal molecular biology and evolution
Year 2026
DOI
10.1093/molbev/msag243
URL
https://doi.org/10.1093/molbev/msag243
Keywords Keywords not found

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