Natural variation in IBF1 confers antimicrobial activity through altered flavonoid accumulation in rice hulls

Clicks: 23
ID: 328844
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #111 of 406 articles by views in Journal of experimental botany

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 406 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract Secondary metabolites perform diverse physiological functions, and studies have identified genes and mechanisms underlying their biosynthesis. However, the genetic basis of intraspecific variation in secondary metabolism remains poorly understood, particularly in less-studied tissues such as rice hulls. In this study, we identified variants in the F-box protein IBF1 that underlie genotypic differences in hull color and flavonoid accumulation using positional cloning. While IR64 (straw-white hull) harbors the functional IBF1, DJ123 (pigmented hull) harbors frame-shift mutations that result in the loss of a Kelch domain. Yeast two-hybrid analysis showed that all three Kelch domains of IBF1 are required for interaction with chalcone synthase 1 (CHS1). Accordingly, the DJ123 variant (IBF1DJ123) does not interact with CHS1. This finding was further supported by deep learning-based structural modeling. An IR64-based chromosomal segment substitution line (CSSL) carrying IBF1DJ123 showed increased flavonoid content and reduced expression of CAD, a gene involved in lignin synthesis, compared to IR64. Metabolites in the CSSL suppressed the growth and siderophore generation activity of Pantoea species, which can act as beneficial or pathogenic endophytes. This study highlights the impact of a single gene on diverse metabolite accumulation patterns and suggests that such variation may be exploited for defense against pathogens.
Reference Key
openalex_W7213405261 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Yoshiaki Ueda, Yoshinori Murata, Nozomu Sakurai, T. Ogata, Hiroki Saito, Juan Pariasca Tanaka, Katsuhiko Kondo, Hideki Takanashi, Takuma Ishizaki, Yasunari Fujita, Matthias Wissuwa
Are you one of these authors? Claim your profile
Journal Journal of experimental botany
Year 2026
DOI
10.1093/jxb/erag461
URL
https://academic.oup.com/jxb/advance-article-pdf/doi/10.1093/jxb/erag461/71125911/erag461.pdf
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.