Selective curing of the endogenous plasmid pSF3 encoding organophosphorus hydrolase in Sphingobium fuliginis ATCC 27551

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ID: 328829
2026
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Abstract
Sphingobium fuliginis ATCC 27551 degrades organophosphorus pollutants by organophosphorus hydrolase (OPH) encoded on a plasmid pSF3. The replacement plasmid with the same replication-associated genes was used to cure pSF3. By the selection of antibiotic resistance, pSF3 was eliminated without changing other plasmids. The cured strain would be useful to construct a strain capable of large-scale production of OPH.
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openalex_W7213350040 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Satoshi Ishikawa, Natsumi Ishikawa, Satoshi Onodera, Yuji Kikuchi, Kazuyoshi Kawahara
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Journal bioscience biotechnology and biochemistry
Year 2026
DOI
10.1093/bbb/zbag141
URL
https://doi.org/10.1093/bbb/zbag141
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