Heterologous Surface Display of Serratia marcescens -Derived Laccase and Its Decolorization Performance toward Azo Dyes

Clicks: 28
ID: 328225
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #108 of 220 articles by views in Journal of applied microbiology

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 220 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
AIMS: Laccase represents a high-value green catalyst with extensive industrial application prospects. The aim of this study is to construct a laccase surface-displaying recombinant strain LacA-BL21 by fusing LacA from Serratia marcescens with Escherichia coli (E. coli) Ag43 autotransport system, optimize its enzyme production and dye decolorization parameters, verify the surface display system efficiency, and evaluate its potential for industrial wastewater bioremediation. METHODS AND RESULTS: In this study, we generated the surface-displaying strain LacA-BL21 by fusing laccase (LacA) from S. marcescens with the Ag43 autotransport system from E. coli. The enzyme activity results indicate that the recombinant laccase exhibits optimal catalytic activity at 45°C and pH 4.0. Key parameters affecting enzyme production were systematically optimized and LacA-BL21 achieved a maximum laccase activity of 43.66 ± 1.45 U mL-1 in TB medium supplemented with 4% methanol, 1.77 times that of the wild-type strain ZH-5. Furthermore, qRT-PCR analysis demonstrated that the relative transcription level of the target gene in the LacA-BL21 was 1.91-fold higher than that in the wild-type strain ZH-5, verifying the high efficiency of the surface display system for laccase heterologous expression. Dye decolorization assays using simulated wastewater were performed to evaluate the catalytic performance of recombinant LacA-BL21 system, and the results indicated outstanding decolorization efficacy toward various dye substrates. Following systematic process optimization, the crude preparation containing recombinant LacA achieved an 82.17% decolorization rate for Congo red simulated wastewater within 2 hours. CONCLUSION: This study constructs a surface display system for laccase expression, which exhibits exceptional catalytic efficiency and favorable decolorization capability toward azo dyes, thereby harboring substantial promise for the bioremediation of industrial wastewater.
Reference Key
openalex_W7212147444 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Fumin Tan, Ying Pan, Qifeng Li, Enguo Fan, Cuijuan Gao
Are you one of these authors? Claim your profile
Journal Journal of applied microbiology
Year 2026
DOI
10.1093/jambio/lxag232
URL
https://doi.org/10.1093/jambio/lxag232
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.