Haplotype-specific expression of a terpene synthase underlies linalool variation in the grapevine cultivar Riesling

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ID: 327855
2026
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Abstract
Grapevine cultivars vary widely in monoterpenoid content, yet the genetic and regulatory mechanisms underlying this variation remain poorly characterized beyond highly aromatic Muscat types. We profiled free volatiles and monoterpenoid glycosides in a Riesling × Cabernet Sauvignon F1 mapping population, revealing extensive variation and transgressive segregation consistent with multigenic control. QTL mapping identified 70 significant loci associated with 48 volatile compounds and monoterpene glycosides, including two major QTLs explaining 33.6% and 33.4% of phenotypic variance in (3S)-linalool accumulation. Integration of haplotype-resolved transcriptomics with metabolite data, enabled by a chromosome-scale diploid Riesling genome assembly, resolved a (3S)-linalool/nerolidol synthase cluster on chromosome 10 and identified VviTPS54 as the strongest candidate underlying linalool variation. VviTPS54 exhibited haplotype-specific expression strongly correlated with (3S)-linalool accumulation across genotypes, while no QTL was detected at the 1-deoxy-D-xylulose-5-phosphate synthase 1 (VviDXS1) locus previously identified in Muscat cultivars. In addition, VviDXS1 expression was not correlated with terpene levels, indicating that regulatory variation within terpene synthase clusters, rather than methylerythritol phosphate (MEP) pathway flux, drives monoterpenoid composition in this population. These results establish regulatory variation of terpene synthases as a key mechanism underlying monoterpenoid diversity in grapevine and demonstrate that resolving such variation requires haplotype-phased genome assemblies coupled with haplotype-resolved transcriptomics to detect allele-specific expression differences at complex, heterozygous loci.
Reference Key
openalex_W7160318122 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Jerry Lin, Mélanie Massonnet, Noé Cochetel, Larry Lerno, Luis Díaz García, Mireia Domenech-Lopez, Rosa L. Figueroa, Susan E. Ebeler, Dario Cantu
Journal Journal of experimental botany
Year 2026
DOI
10.1093/jxb/erag448
URL
https://doi.org/10.1093/jxb/erag448
Keywords Keywords not found

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