Limb restraint induces a rapid and profound decrease in core body temperature associated with the stress response in mice

Clicks: 15
ID: 327664
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #126 of 151 articles by views in PNAS nexus

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 151 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract Stress responses are triggered by diverse stressors and typically involve acute common physiological changes including increased heart rate and adrenocortical hormone secretion. In contrast, core body temperature (Tc) may either increase or decrease depending on the stressor. Although restraint is widely used to induce stress responses in laboratory animals, heterogeneous effects on Tc have been reported. To elucidate the central and peripheral mechanisms underlying restraint-induced hypothermia, a reliable model that consistently induces hypothermia is first required. In this study, we aimed to establish an experimental system using mice that can reliably induce hypothermia through physical restraint. Furthermore, we aimed to elucidate the primary factors contributing to hypothermia within the various elements constituting the restrained state and to simplify the restraint experimental system by eliminating unnecessary elements. Starting with conventional cylinder-shaped restraint equipment and modifying it in various ways, we finally found that the simple procedure of limb restraint reliably induces a rapid (within 15 min) and substantial (approximately 4 °C) decrease in rectal temperature, a good indicator of Tc. Limb restraint increased serum corticosterone concentration and activated the paraventricular hypothalamic nucleus, suggesting that a type of stress response involving Tc reduction was induced by limb restraint. The decrease in body temperature caused by limb restraint was attributed, at least in part, to reduced heat production in the brown adipose tissue (BAT) and increased heat loss from the tail. This simplified restraint paradigm provides a useful tool for elucidating the mechanisms underlying hypothermia induced by physical restraint.
Reference Key
openalex_W7208773289 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Masashi Watanave, Rei Izumi, Momoka Aizaki, Takumi Hatai, Naoi Sakaguchi, Sakura Hayashi, Miho Ashikawa, Megumi Eguchi, Hayato Umehara, Shun Yamaguchi
Are you one of these authors? Claim your profile
Journal PNAS nexus
Year 2026
DOI
10.1093/pnasnexus/pgag301
URL
https://doi.org/10.1093/pnasnexus/pgag301
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.