MAD2B guides chromosome stability in mitosis via the MAD2B–CIP2A signaling axis
Clicks: 11
ID: 327278
2026
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Emerging Content
3.0
/100
11 views
10 readers
AI Quality Assessment
Not analyzed
Readership in this journal
EmergingRanked #54 of 56 articles by views in journal of molecular cell biology
Most read
Least read
Bar heights use a square-root scale.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Error-free mitosis depends on accurate chromosome attachment to spindle microtubules, monitored by the spindle assembly checkpoint machinery, which prevents precocious chromosome segregation and reduces the risk of aneuploidy. MAD2B, a homologue of MAD2, is critical for mitotic quality control and DNA damage repair. However, it remains elusive how MAD2B guides genome surveillance during the cell cycle. Here, we show that MAD2B safeguards genome integrity through interactions with CIP2A in a context-dependent manner. To delineate the molecular mechanisms underlying MAD2B-dependent signaling in mitosis, we carried out affinity purification of FLAG-MAD2B followed by mass spectrometry to identify MAD2B-associated proteins. Our biochemical characterization uncovered a previously uncharacterized interaction between MAD2B and CIP2A, mediated by the N-terminus of CIP2A. Importantly, MAD2B depletion resulted in DNA damage response and replication stress phenotypes, leading to aberrant mitotic DNA synthesis primarily in HeLa cells. Notably, loss of MAD2B disrupted CIP2A recruitment to γH2AX-marked DNA lesions and attenuated DNA damage repair. Together, these results establish MAD2B as a context-sensitive regulator of genome stability that links replication stress surveillance to mitotic chromosome repair via the MAD2B-CIP2A signaling axis.
| Reference Key |
openalex_W7204628765
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | Umer Ejaz, Fangyuan Xiong, Hengyi Shao, Tahir Ullah, Xuebiao Yao, Liangyu Zhang, Zhen Dou |
| Journal | journal of molecular cell biology |
| Year | 2026 |
| DOI |
10.1093/jmcb/mjag034
|
| URL | |
| Keywords | Keywords not found |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.