Development of a defined three-strain nitrifying microbial community for ammonification and nitrification under organic nitrogen conditions

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ID: 327277
2026
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Abstract
A simplified synthetic nitrifying microbial community capable of sequential ammonification and nitrification under organic conditions was constructed using three defined strains: Bacillus badius NBRC 15713, Nitrosomonas europaea NBRC 14298, and Nitrobacter winogradskyi NBRC 14297. This co-culture achieved complete ammonification and nitrification under selected organic nitrogen conditions, with NO₃⁻-N accumulation of approximately 56 mg-N/L and no residual NO₂⁻-N detected. RT-qPCR analysis showed that changes in the relative abundance of N. europaea and N. winogradskyi were associated with changes in inorganic nitrogen concentrations during cultivation, indicating that the co-culture supported the growth and activity of nitrifying bacteria despite the presence of organic compounds that inhibit nitrification. This defined three-strain community provides a reproducible model for studying sequential microbial nitrogen conversion under organic nitrogen conditions.
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openalex_W7204628697 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Htun Inzali, Sakuntala Saijai, Wakako Okada, Jun Ogawa, Akinori Ando
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Journal bioscience biotechnology and biochemistry
Year 2026
DOI
10.1093/bbb/zbag131
URL
https://doi.org/10.1093/bbb/zbag131
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