103 A Medulloblastoma brain slice culture radiotherapy platform to dually assess therapeutic efficacy and toxic

Clicks: 19
ID: 326724
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #158 of 287 articles by views in journal of neuro-oncology

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 287 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract Introduction Medulloblastoma is the most common malignant childhood brain tumour. Radiotherapy (RT) forms a key treatment pillar but is associated with significant late effects. Ex vivo brain slice cultures present a translational model that overcomes limitations of existing in vitro and in vivo alternatives. Slice cultures provide a native “scaffold”, with representative cellular architecture and extracellular matrix (ECM), upon which cancer cell growth and invasion can be tracked in real-time. We describe an organotypic brain slice culture (OSC) model for Group3 medulloblastoma (MBGrp3), amenable to long-term culture and irradiation. Method MBGrp3 spheroids (g-MYC-tdTomato) were cultured to an optimised diameter of 250-350µm in ultra-low-attachment plates. Murine cerebellar OSCs were prepared using a vibratome and cultured on transwell inserts. Co-cultures were established at 72-hours, and RT delivered 48 hours later using a Small-Animal-Radiation-Research-Platform (SARRP). 1, 3 or 5 daily fractions (1.8Gy) or a single cumulative dose (9Gy) was delivered. Tissue toxicity was assessed through live/dead staining and ToxiLight® assay. Growth and invasion was quantified in real-time (live multi-photon-microscopy) and spheroid volume/invasion analysed (Huygen’s). OSCs were fixed at endpoint for confocal microscopy to assess DNA damage (γH2AX) and cellular architecture. Results Control OSCs maintained viability long-term (21-days) with preserved cellular architecture and ECM. Live/dead staining revealed dose-dependent tissue toxicity following RT, with 5 fractions or a single cumulative dose significantly reducing viability (p < 0.05). DNA damage accumulated over time in culture and was dose-dependent. Our multi-photon pipeline provided repeat live volumetric quantification of spheroid growth. Conclusions Fractionated RT of OSCs is feasible, demonstrating dose-dependent DNA damage. This model serves as a basis for future studies to test radio-sensitising agents wherein cancer cell growth and invasion can be modelled in tandem with tissue toxicity.
Reference Key
openalex_W7204455629 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kristy Kehoe, Louisa Taylor, Melanie Beckett, Glyn Nelson, Samantha Jepson Gosling, Janice Law, Mike Tseung, Connie Mackenzie-Gray Scott, Christopher Cowie, Fiona LeBeau, Rebecca Hill, Steve Clifford
Are you one of these authors? Claim your profile
Journal journal of neuro-oncology
Year 2026
DOI
10.1093/neuonc/noag172.035
URL
https://academic.oup.com/neuro-oncology/article-pdf/28/Supplement_1/noag172.035/70805794/noag172.035.pdf
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.