Acute Exposure to Perfluorooctanoic Acid (PFOA) During Cardiomyogenesis disrupts Transcriptional and Electrophysiological Profiles in Differentiated Myocytes

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ID: 326157
2026
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Ranked #80 of 153 articles by views in toxicological sciences : an official journal of the society of toxicology

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Abstract
Abstract Exposure to per and polyfluoroalkyl substances (PFAS) are linked to various cardiovascular diseases (CVDs), but effects of developmental PFAS exposures on the human heart remain unclear. Using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM), the objective of this study was to investigate the effects of PFAS exposure during cardiac differentiation on gene expression and function of cardiomyocytes. We exposed two hiPSC lines (one male and one female donor) to perfluorooctanoic acid (PFOA), a common and ubiquitous PFAS (0.05, 0.5, 5, 50, 100, 150, 200 μM), followed by assessment of cellular number and pluripotency marker expression. PFOA exposure for 72 hours had no significant effects on hiPSC pluripotency, and modest inhibition of proliferation was observed only at the highest concentration. hiPSCs were then differentiated into ventricular cardiomyocytes in the continued presence or absence of PFOA using an established protocol. Optical mapping studies revealed dose and cell line-specific effects of PFOA on cardiomyocyte voltage and calcium dynamics that were still present 10 days after cessation of exposure. Patch clamping studies demonstrated small but significant reductions in repolarizing IKr currents with 5µM PFOA exposure in cardiomyocytes from both donors. Using RNA-seq, we found that exposure to PFOA led to significant changes in transcriptional pathways related to lipids and lipoproteins in the female hiPSC-CM, and developmental pathways and calcium homeostasis in the male hiPSC-CM. These findings provide direct experimental evidence that transient developmental exposure to PFOA can durably reprogram human cardiomyocyte gene expression and function, supporting a developmental origin of PFAS-associated cardiovascular disease risk.
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Authors Tomoko Ishikawa, Christopher Wayne Clark, Anagha Tapaswi, Kimberley E. Sala-Hamrick, Todd J. Herron, Eric N. Jiménez‐Vázquez, Abhilasha Jain, D.r.t. Jones, Justin Colacino, André Monteiro da Rocha, Laurie K. Svoboda
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Journal toxicological sciences : an official journal of the society of toxicology
Year 2026
DOI
10.1093/toxsci/kfag107
URL
https://doi.org/10.1093/toxsci/kfag107
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