cis- and trans -regulatory factors contributing to divergent activity of the TDH3 promoter in Saccharomyces yeast

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ID: 324725
2026
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Abstract
Abstract Changes in regulatory sequences controlling the timing and activity of gene products underlie much of natural phenotypic variation. Yet, identifying which divergent sites matter and how they impact gene expression remain challenging. Here, we investigate how transcriptional activity and homeostatic responsiveness of orthologous promoters of the metabolic gene TDH3 evolved among Saccharomyces yeast. We found that promoter expression level increased specifically in the S. cerevisiae lineage and that a substantial part of this increase was caused by genetic variants located between the well-characterized, conserved binding sites for two direct transcriptional regulators. These nucleotide changes altered the promoters’ expression levels while leaving the expression dynamics conserved. Further, the effects of these nucleotide changes were only seen in the presence of a third transcription factor, TYE7p, which is known to be recruited by the other transcription factors through protein-protein interactions. These results suggest that the cis-regulatory changes act through their influence on the collective assembly/activation of a transcription factor complex, and that changes acting through such a mechanism can allow distinct parts of gene expression, such as expression level and dynamics, to separately diverge.
Reference Key
openalex_W7202277690 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Mohammad A. Siddiq, Hannah Kania, Nicholas J Brown, Patricia J. Wittkopp
Journal molecular biology and evolution
Year 2026
DOI
10.1093/molbev/msag203
URL
https://doi.org/10.1093/molbev/msag203
Keywords Keywords not found

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