Reprogramming a Hgt-family Kluyveromyces marxianus sugar transporter by site-directed mutagenesis to enable co-consumption of glucose and xylose

Clicks: 5
ID: 324341
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal

Ranked #29 of 38 articles by views in fems yeast research

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract Efficient utilization of lignocellulosic hydrolysates in yeast-based biorefineries requires simultaneous consumption of glucose and xylose, which is often limited by preferential glucose uptake. In Kluyveromyces marxianus, we kinetically characterized two native xylose transporters, KMAR_10 531 and KMAR_60 179, identifying medium- and low-affinity xylose transporters, respectively. KMAR_10 531 also mediated high-affinity glucose uptake (Km 0.28 ± 0.1 mM), limiting xylose utilization in mixed-sugar media. Guided by structural modelling, we engineered the KMAR_10 531 N325V variant, which reduced glucose affinity ~20-fold while improving xylose affinity more than 3-fold (Km reduced from 46.9 ± 9.5 to 14.9 ± 3.6 mM). Expression of KMAR_10 531 N325V in a pentose-transporter-deficient K. marxianus strain enabled simultaneous glucose–xylose co-consumption in flasks and bioreactors, overcoming the diauxic growth observed with the native transporter. In bioreactors, the engineered strain consumed ~90% of available xylose within 45 h and produced increased biomass compared to native transporter. This study provides the first example of engineering an Hgt-like transporter for altered sugar specificity.
Reference Key
openalex_W7201988095 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Lorena Donzella, Carlos Belloch-Molina, John P. Morrissey, Maria João Sousa
Journal fems yeast research
Year 2026
DOI
10.1093/femsyr/foag035
URL
https://doi.org/10.1093/femsyr/foag035
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.