Purification and biochemical characterization of an extracellular endo xylanase from Phlebia acerina SF23754

Clicks: 24
ID: 323798
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #46 of 99 articles by views in bioscience biotechnology and biochemistry

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract Efficient lignocellulose conversion requires xylan-degrading enzymes that function without energy-intensive pretreatment. Although many xylanases have been reported, the biochemical diversity of Basidiomycota-derived enzymes remains unclear. Here, we purified and characterized the extracellular xylanase secreted by Phlebia acerina SF23754. The enzyme is a 44-kDa protein with an acidic pH optimum (pH 4.0) and an optimal temperature of 50 °C. It retained over 80% activity after 24 h at pH 3–9, showed moderate thermal stability, and was strongly inhibited by heavy metals, whereas EDTA had no effect. Tryptic peptides mapped to conserved GH10 motifs, identifying the enzyme as a basidiomycete GH10 xylanase. It hydrolyzed glucuronoxylans and arabinoxylans to produce short xylooligosaccharides without releasing xylose. Removal of arabinose substituents markedly enhanced arabinoxylan degradation, indicating a preference for less substituted xylan regions. These features highlight a GH10-type catalytic strategy in Basidiomycota and broaden understanding of fungal xylan degradation mechanisms.
Reference Key
openalex_W7172520081 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kenji Okamoto, Takato Yasutake, Daiki Hosoo, Toshifumi Mizuta, Tatsuya Kondo, Tatsuji Sakamoto
Are you one of these authors? Claim your profile
Journal bioscience biotechnology and biochemistry
Year 2026
DOI
10.1093/bbb/zbag120
URL
https://doi.org/10.1093/bbb/zbag120
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.