Re-evaluation of the Anti-glycation Activity of Herbal Extracts Using Multiple Anti-glycation Assays

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ID: 322650
2026
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Abstract
Abstract The glycation process in vivo, including early intermediates, advanced glycation end products (AGEs), and crosslinked species, is involved in diabetes-associated tissue damage. Although some herbal extracts have been reported to exert antidiabetic or anti-glycation effects, most studies have used co-addition assays to evaluate the inhibition of AGE formation. We re-evaluated eight herbal extracts using both co-addition and post-addition assays in distinct glycation models, namely glyceraldehyde-induced collagen and lysozyme glycation models. In the collagen model, Coptis, Cinnamomum, Connarus, and Pueraria reduced AGE-derived fluorescence under both co-addition and post-addition assays. In the lysozyme post-addition model, Cinnamomum, Connarus, and Pueraria reduced the dimer band, whereas Coptis showed a more limited effect. Multiple anti-glycation assays helped distinguish inhibition of the formation of glycation-related modifications from effects on preformed glycation-related modifications and revealed distinct anti-glycation profiles among the herbal extracts. Our results highlight the value of multiple glycation assays for anti-glycation profiling of herbal extracts.
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Authors Mami Ishikawa, Rika Taniguchi, Taizo Taniguchi, Zhao Yang, Takahito Nishikata
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Journal bioscience biotechnology and biochemistry
Year 2026
DOI
10.1093/bbb/zbag118
URL
https://doi.org/10.1093/bbb/zbag118
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