Pre-exposure to phosphate-buffer saline (PBS) renders some Pseudomonas aeruginosa strains susceptible to triclosan on solid medium

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ID: 322275
2026
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Abstract
Triclosan is an antibiotic frequently used to selectively isolate Pseudomonas aeruginosa from environmental and animal samples. Here, we report that while nine tested P. aeruginosa strains were indeed triclosan-resistant when grown on Luria Bertani (Lennox) broth agar supplemented with triclosan, all nine exhibited decreased CFU by plating following pre-exposure to phosphate-buffer saline (PBS). Compared to growth on lysogeny broth agar in the absence of PBS incubation, PBS pre-exposure was associated with mean CFU reductions ranging from ∼25% (clinical isolate PABL048) to ∼80% (laboratory strain PA14). The potential for triclosan agar to cause significant errors in experiments designed to quantify P. aeruginosa numbers was demonstrated using a mouse model of P. aeruginosa gastrointestinal carriage. In this experiment, P. aeruginosa carriage was underestimated by up to 20-fold when fecal samples were processed using PBS. These findings suggest that triclosan should be used with caution to quantify P. aeruginosa numbers in protocols that utilize PBS.
Reference Key
openalex_W7170343302 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Marine Lebrun-Corbin, Somalisa Pan, Preeti Garai, Alan R. Hauser
Journal FEMS microbiology letters
Year 2026
DOI
10.1093/femsle/fnag086
URL
https://doi.org/10.1093/femsle/fnag086
Keywords Keywords not found

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