Identification and Transcription Analysis of Phenolic Acid Biosynthesis Pathway in Pseudotaxus chienii

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ID: 321184
2026
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Abstract
Abstract Pseudotaxus chienii, an endangered relic conifer endemic to China, possesses significant medicinal potential, yet its phenolic acid biosynthesis and glycosylation mechanisms remain unelucidated. This study integrated metabolomics, mass spectrometry imaging (MSI), transcriptomics, and molecular biology to systematically characterize the phenolic acid pathway in P. chienii. Untargeted metabolomics identified distinct phenolic acid accumulation patterns between P. chienii and Taxus mairei. MALDI-2 MSI visualized ten phenolic acids, confirming leaves as the primary accumulation site. Genome-wide analysis revealed a complete phenolic acid biosynthesis pathway with key enzyme-encoding genes (4 PAL, 5 CCR, 2 F5H, etc.), while HQT was absent, indicating loss of chlorogenic acid synthesis. We identified 504 glycosyltransferase (GT) genes, with 48 leaf-specific ones (predominantly GT1 subfamily). Promoter and correlation analyses highlighted WRKY (PichiChr12G332970.1) and ERF (PichiChr3G086100.1) as core transcription factors (TFs). EMSA and dual-luciferase assays validated WRKY(PichiChr12G332970.1) directly activating three GT genes. This study clarifies the molecular basis and transcriptional regulation of phenolic acid glycosylation in P. chienii, providing a framework for exploiting its medicinal resources and guiding conservation-oriented breeding.
Reference Key
openalex_W7169182215 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Xiaori Zhan, Zijing Zhou, Yuting Peng, Xinru Wei, Zixin Shao, 胡艳恒, Yanjun Yang, Chenjia Shen
Journal plant and cell physiology
Year 2026
DOI
10.1093/pcp/pcag097
URL
https://doi.org/10.1093/pcp/pcag097
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