nf-core/magmap: Map metatranscriptomes to large collections of genomes

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ID: 320533
2026
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Abstract
Abstract Summary The lack of publicly available reference genomes has forced annotation of metatranscriptomes to either use direct alignment of sequence reads to reference databases or de novo assembly. As more and more natural environments are covered by metagenomic surveys, this is rapidly changing. This opens up the possibility of genome-resolved studies of prokaryotic metatranscriptomes by mapping to genomes from public repositories or metagenome-assembled genomes derived from the same environment. Here, we present the nf-core/magmap pipeline that provides a reproducible, easy-to-access, and well-documented workflow for selecting reference genomes, mapping to them, and quantifying features. Genomes can be drawn from public sources or originate from private collections. The pipeline is primarily aimed at prokaryotic communities but can, together with collections of reference mature gene sequences, also be applied to eukaryotes. Availability and implementation The nf-core/magmap pipeline is implemented in Nextflow and part of the nf-core collaboration. The pipeline is available at the nf-core website (https://nf-co.re/magmap) and GitHub (https://github.com/nf-core/magmap). Supplementary information Supplementary data are available at Bioinformatics online.
Reference Key
openalex_W7167912497 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Danilo Di Leo, Emelie Nilsson, George Westmeijer, Jarone Pinhassi, Daniel Lundin
Journal BMC Bioinformatics
Year 2026
DOI
10.1093/bioinformatics/btag501
URL
https://doi.org/10.1093/bioinformatics/btag501
Keywords Keywords not found

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