Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies.

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ID: 32038
2019
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Abstract
Sickle cell disease (SCD) is one of the most prevalent and severe monogenetic disorders. Previously, we generated iPS cell lines from SCD patients. Here, we generated iPS cell lines from three age-, ethnicity- and gender-matched healthy individuals as control cell lines. Cell reprogramming was performed using erythroblasts expanded from PBMC by a non-integrative method. SCD-iPSC controls expressed pluripotency markers, presented a normal karyotype, were able to differentiate into the three germ layers in embryoid body spontaneous differentiation and confirmed to be integration-free. The cell lines generated here may be used as matched healthy controls for SCD studies.
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paredes2019generationstem Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Paredes, Bruno Diaz;Martins, Gabriele Louise Soares;Azevedo, Carine Machado;Sampaio, Gabriela Louise de Almeida;Nonaka, Carolina Kymie Vasques;Silva, Katia Nunes da;Soares, Milena Botelho Pereira;Santos, Ricardo Ribeiro Dos;Souza, Bruno Solano de Freitas;
Journal Stem cell research
Year 2019
DOI
S1873-5061(19)30084-4
URL
http://www.sciencedirect.com/science/article/pii/S1873506119300844
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