Evaluation and mutagenesis of an assimilatory nitrate reduction bacterium Priestia megaterium BZ-95

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ID: 319238
2026
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Abstract
Abstract Aims Excessive NO3−-N and NO2−-N accumulation threatens aquaculture water quality, highlighting the need for aerobic microorganisms capable of converting dissolved inorganic nitrogen into biomass-associated organic nitrogen. This study aimed to characterize the assimilatory NO3−/NO2− reduction mechanism of Priestia megaterium BZ-95 and to evaluate its potential for aquaculture wastewater treatment. Methods and Results A NO3−-reducing bacterium, P. megaterium BZ-95, was investigated using whole-genome analysis, gene expression profiling, and nitrogen-removal assays. Genome analysis revealed two nitrogen-assimilation modules, namely a NO3− assimilation cluster (nasC-nirB_2-narT) and a nitrite assimilation operon (nirB_1-nirD-nirC-cysG), where as no denitrification or DNRA genes were detected. In NO3−-N medium, NO3−-N decreased from 72.59 to 8.07 mg/L within 24 h, accompanied by transient NH4+ accumulation. In NO2−-N medium, NO2−-N decreased from 70.00 to 0.009 mg/L within 21 h, with no detectable NH4+ accumulation. In mixed-N medium, NO3−-N and NO2−-N decreased from 30.00 to 3.33 mg/L and from 30.00 to 0.99 mg/L, respectively, within 24 h. Gene expression analysis showed that NO3− mainly induced the nasC-nirB_2-narT module, whereas NO2− strongly induced the nirB_1-nirD-nirC-cysG module. In addition, the mutant BZ-95D4 showed a 61.08% higher NO3−-N removal capacity than the parental strain BZ-95. Conclusions P. megaterium BZ-95 possesses a dual-module assimilatory nitrogen-reduction system with distinct responses to NO3− and NO2−. These findings improve understanding of microbial nitrate assimilation and indicate that BZ-95, particularly the improved strain BZ-95D4, is a promising candidate for aquaculture wastewater treatment.
Reference Key
openalex_W7166817296 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Hao Chen Jiang, Xian Cong Fan, Ke Zheng, Yan Zhao, Zi Yan Jin, Yu Cheng, Yan Zou, Xiang Shan Ji
Journal Journal of applied microbiology
Year 2026
DOI
10.1093/jambio/lxag159
URL
https://doi.org/10.1093/jambio/lxag159
Keywords Keywords not found

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