Discovery proteomics identification of factors contributing to gonadotropin β expression
Clicks: 22
ID: 318739
2026
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Emerging Content
6.3
/100
22 views
10 readers
AI Quality Assessment
Not analyzed
Readership in this journal
EmergingRanked #198 of 311 articles by views in american journal of physiology endocrinology and metabolism
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 311 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Luteinizing hormone (LH) and follicle-stimulating hormone (FSH), critical for reproduction, are heterodimers of a common α subunit and unique β subunits, that are limiting components in the mature hormone synthesis. GnRH from the hypothalamus regulates synthesis of LH and FSH β-subunits. Previous studies identified signaling pathways and transcription factors that mediate GnRH induction of β-subunits. However, these studies do not fully explain gonadotrope responsiveness to GnRH or mechanisms of how a single hormone can regulate two β-subunits differentially. We postulated additional transcription factors, coactivators or corepressors, that are awaiting identification. To provide novel candidates, we used discovery proteomics of protein complexes that associate with gonadotropin β promoters regions that contain GnRH-responsive elements, and protein complexes that interact with transcription factors that are activated by GnRH to induce gonadotropin β genes. Data are available via ProteomeXchange with identifier PXD075636. Using computational tools, such as Intervene and Tidyproteomics, we compare and contrast proteins in complexes to identify coactivators or corepressors that may be unique or in common for both gonadotropin β subunit transcriptional upregulation or repression, novel transcription factors, and proteins that may regulate both gonadotropin β gene expression, proteins specific to each promoter, and proteins that are recruited via interaction with intermediate GnRH-induced transcription factors. Our studies can serve as a resource to interrogate potential candidates in cell cultures and in vivo for their roles in gonadotropin β expression. Understanding the mechanisms whereby GnRH regulates gonadotropin hormone levels will provide insight into the physiology and pathophysiology of the reproductive system.
| Reference Key |
openalex_W7165931967
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | Rebecca Elizabeth Ruggiero-Ruff, Brandon H. Le, Djurdjica Coss |
| Journal | american journal of physiology endocrinology and metabolism |
| Year | 2026 |
| DOI |
10.1210/endocr/bqag073
|
| URL | |
| Keywords | Keywords not found |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.