Neurite Outgrowth–Promoting Furanocoumarins Isolated from Sudachi Juice in PC12 Cells

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ID: 318499
2026
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Abstract
Abstract Sudachi (Citrus sudachi, a citrus fruit of the Rutaceae family) juice was found to promote neurite outgrowth in PC12 cells. Activity-guided purification of the juice led to the isolation of two furanocoumarins, byakangelicin and isopimpinellin, and a flavone, demethoxysudachitin, identifying them as neurite-outgrowth promoters for the first time. Byakangelicin and isopimpinellin showed markedly stronger neurite outgrowth–promoting activity than demethoxysudachitin, prompting a comparative evaluation of five furanocoumarins, including both isolated and related reference compounds. The results showed that the basic furanocoumarin scaffold alone is inactive, whereas substitution at either the C5 or C8 position of a furanocoumarin confers prominent activity, with dual substitution further improving activity. Among the tested compounds, byakangelicin and isopimpinellin exhibited the most potent neurite outgrowth–promoting activity. These findings identify sudachi-derived furanocoumarins as previously unrecognized neurite-outgrowth promoters and suggest their potential as neurotrophic agents for supporting cognitive function.
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openalex_W7165684706 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Saki Morimoto, Takeru Koga, Saho Miyazaki, Akihiro Tai
Journal bioscience biotechnology and biochemistry
Year 2026
DOI
10.1093/bbb/zbag092
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