MULTIDIMENSIONAL SPATIAL MAPPING OF EXTRACELLULAR MATRIX: CARTILAGINOUS-OSSEOUS COMPOSITE FORMATION, TENDON INTEGRATION AND VASCULARIZATION DURING SKELETAL GROWTH AND REPAIR
Clicks: 5
ID: 318069
2026
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Emerging Content
0.6
/100
5 views
2 readers
AI Quality Assessment
Not analyzed
Readership in this journal
EmergingRanked #65 of 120 articles by views in journal of bone and mineral research : the official journal of the american society for bone and mineral research
Most read
Least read
Bar heights use a square-root scale.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Abstract The extracellular matrix provides a crucial tissue-specific signaling hub and structural scaffold that transduces biomechanical force load into cellular responses. While this is especially important in the musculoskeletal joint, the spatial organization of extracellular matrix (ECM) assemblies has been poorly investigated. Dense supra-structures, inaccessible epitopes and complex antigen retrieval negatively affect the 3D-visualization of ECM-scaffolds in connective tissues. We have developed multiplex ECM immuno-staining techniques that now permit mapping of the entire musculoskeletal ECM with embedded cells during skeletal growth and repair. Specifically, we show that osteoblasts deposit a bony ECM on the cartilaginous template in the hypertrophic growth plate, creating a unique ECM composite that likely confers both stiffness and flexibility to neonatal bone. We demonstrate that tendon collagen I fibrils anchor along a thin, continuous tidemark at the lateral cartilaginous surface, mechanically coupling cartilage and tendon matrices at contact sites. Finally, we reveal muscle-derived laminin γ1(+) vascular basement membrane infiltration of the cartilaginous fracture callus ECM, likely facilitating nutrient delivery and structural stabilization during skeletal repair. These advances provide insight into the complexity of the ECM and its interplay with embedded cell clusters at previously unattainable spatial resolution and biological context during skeletal growth and repair.
| Reference Key |
openalex_W7165458784
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | Julia Etich, Kristina Probst, Jan Miller, Denise Meinberger, Kristina Bubb, Marcus Krüger, Manuel Koch, Frank Zaucke, Anita Ignatius, Verena Fischer, Yvonne Mödinger, Attila Aszódi, Paolo Alberton, Alvise Schiavinato, Lydia Sorokin, Timm Schroeder, Bent Brachvogel |
| Journal | journal of bone and mineral research : the official journal of the american society for bone and mineral research |
| Year | 2026 |
| DOI |
10.1093/jbmr/zjag077
|
| URL | |
| Keywords | Keywords not found |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.