Disease-specific gut microbial signatures generate model-derived cancer probability scores through targeted fecal qPCR profiling

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ID: 318023
2026
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Abstract
The gut microbiome is a potential source of non-invasive cancer biomarkers. We evaluated six fecal microbial markers and developed targeted qPCR-based logistic models for colorectal cancer (CRC) and pancreatic cancer (PC). Using LASSO with the 1-standard-error rule, four markers were selected for CRC (afb, nan, fsr, and 5ar) and three for PC (but, fsr, and saa). In post-selection leave-one-out cross-validation of fixed model structures, the CRC and PC models yielded AUCs of 0.824 and 0.780, respectively. Fixed-model application yielded AUCs of 0.716 for colorectal adenoma and 0.540 for the pancreatic high-risk group. In an exploratory Early PC versus high-risk comparison, the fecal qPCR score showed a higher AUC point estimate than CA19-9, while the difference was not statistically significant. Overall, the disease-specific model performance and fixed-model behavior across clinically related groups support further evaluation of model-derived cancer probability scores as exploratory cancer-assessment tools.
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openalex_W7165391455 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Tadashi Fujii, Eizaburo Ohno, Naoko Nakano, Kazunori Nakaoka, Hideaki Takahashi, Kohei Funasaka, Yohei Doi, Yoshiki Hirooka, Takumi Tochio
Journal bioscience biotechnology and biochemistry
Year 2026
DOI
10.1093/bbb/zbag090
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