Expanding the scope of precision editing in seaweeds through the application of a novel CRISPR-Associated Nuclease 12a (Cas12a) -aligned CRISPR system in Ulva prolifera
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ID: 317932
2026
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Abstract
Seaweeds, such as the fast-growing green alga Ulva prolifera, can be harnessed as valuable marine crops. The lack of scalable genome-editing tools hampers functional genomics to explore and elucidate algal molecular pathways with industrial importance. Here, we expanded precision genome modification in seaweeds by successfully demonstrating gene editing with a transgene-free AT-rich-targeting Cas system in U. prolifera. By evaluating various delivery buffers, comparing different Cas systems, and optimizing incubation temperatures, we determined suitable conditions for higher applicability of a novel Cas12a-aligned ST8 editor. We obtained more than 50 ST8-mediated knock-out mutants of a toxin-based endogenous marker gene, UpAPT, at 28℃ post-delivery incubations. Our work diversified the applicable genome editing tools in seaweeds, advancing algal functional genomics, and providing more strategies to precisely target unexplored seaweed resources.
| Reference Key |
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|---|---|
| Authors | Qin Zheng, Whelver Surnido, Shunya Hozumi, Takamasa Hirano, Shun Sawatsubashi, Hiroyuki Mizuta, Toshiki Uji |
| Journal | Journal of experimental botany |
| Year | 2026 |
| DOI |
10.1093/jxb/erag302
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| URL | |
| Keywords | Keywords not found |
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