A continuous protection strategy using glycan tags to suppress aggregation of hydrophobic peptides

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ID: 317889
2026
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Abstract
Abstract We investigated combination of the glycan tag, peptide-based tag, denaturing agents, and acidic conditions to efficiently prepare highly hydrophobic peptides using E. coli expression. The hydrophilic glycan tag suppressed aggregation of long polypeptides, even when a conventional small ubiquitin-like modifier (SUMO) tag is not sufficient. Moreover, SUMO protease properly recognized and cleaved the SUMO tag in the presence of the additional glycan tag. The finding of the continuous protection strategy enabled efficient preparation of aggregation-prone peptides for semisynthesis of proteins.
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openalex_W7165124031 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Yuta Maki, Youdong Yang, Junpei Abe, Ryo Okamoto, Yasuhiro Kajihara
Journal phytochemistry letters
Year 2026
DOI
10.1093/chemle/upag116
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