Simultaneous single-base resolution profiling of 5mC and 5hmC using BRIGHT-seq

Clicks: 2
ID: 317026
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #273 of 284 articles by views in national science review

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 284 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract Cytosine modifications in DNA, particularly 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC), are key epigenetic marks involved in gene regulation and chromatin organization. Simultaneous and base-resolution detection of 5mC and 5hmC is essential for understanding their interplay in gene regulation. However, existing methods often rely on differential subtraction strategies, making direct and concurrent analysis within a single assay challenging. Here, we present the Base Replacement for Integrated Genome-wide Methylation and Hydroxymethylation Tracking Sequencing (BRIGHT-seq), a sequencing strategy that enables direct and simultaneous detection of 5mC and 5hmC at single-base resolution within the same DNA molecule. By integrating enzymatic and chemical treatments, BRIGHT-seq converts 5mC to adenine and 5hmC to thymine, enabling direct identification of both modifications without relying on differential subtraction. We demonstrate the applicability of BRIGHT-seq by mapping 5mC and 5hmC landscapes across human and mouse genomes. BRIGHT-seq provides a useful and high-resolution tool for epigenetic research, facilitating future studies on the roles of 5mC and 5hmC in gene regulation and disease.
Reference Key
openalex_W7164335076 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Xiaochen Xue, Ziang Lu, Wei Yang, Shaoqing Han, Zhiying Wang, Yifan Jin, Xingxing Li, Xiang Zhou, Yafen Wang, Xiaocheng Weng
Journal national science review
Year 2026
DOI
10.1093/nsr/nwag353
URL
https://doi.org/10.1093/nsr/nwag353
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.