Altered flagellin-TLR5 signaling pathway in interaction between host and laboratory Escherichia coli strains engineered for enhanced intestinal colonization in mouse
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ID: 316931
2026
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Abstract
The intestinal microbiota is essential for maintaining intestinal homeostasis by regulating mucosal immune responses. Identifying bacterial traits that support stable host interactions is therefore crucial for developing next-generation mucosal probiotics. RNase T2 is a conserved ribonuclease with species-specific functions. In Escherichia coli, RNase T2 (RNase I) suppresses biofilm formation, a key process for intestinal colonization. We previously showed that an RNase I-deficient E. coli (Δrna) strain stably colonizes the mouse intestine. Here, we analyzed the immunogenic properties of the Δrna strain. Heat-killed Δrna induced an early IL-1β and TNF-α-skewed cytokine response in RAW264.7 macrophages, with reduced IL-6 expression compared to wild type. In mice, Δrna colonization caused no intestinal tissue injury but enhanced IL-6 and IL-22 expression in the cecum and colon. Selected TLR-specific reporter assays identified TLR5-dependent signaling driven by increased FliC expression. These findings show that RNase I deficiency modulates immune activation while maintaining non-pathogenic mucosal interactions.
| Reference Key |
openalex_W7164157029
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| Authors | Tsuyoshi Tachibana, Atsushi Minami, Ryutaro Fukui, Ryota Tochinai, K. Mochizuki, Wataru Fujii, Tetsuhiro Ogawa, Shigeru Kakuta |
| Journal | bioscience biotechnology and biochemistry |
| Year | 2026 |
| DOI |
10.1093/bbb/zbag079
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| URL | |
| Keywords | Keywords not found |
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