Purine permease 5 contributes to riboflavin distribution in Arabidopsis reproductive organs

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ID: 313730
2026
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Abstract
Riboflavin (vitamin B₂; RF) and its derivatives flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) are indispensable cofactors for redox reactions in plants. While higher plants possess a conserved pathway for de novo riboflavin biosynthesis, how flavins are transported and spatially distributed between tissues remains unresolved. In particular, the molecular identity of plasma membrane transporters involved in flavin transport in plants remains largely unknown. Here, we identify Arabidopsis purine permease 5 (AtPUP5) as a plasma membrane-localized protein associated with riboflavin transport and its distribution in plants. Using a riboflavin-auxotrophic yeast mutant, we show that AtPUP5 enhances intracellular accumulation of RF and, to a lesser extent, FMN, whereas FAD accumulation is inefficient under the conditions tested. In planta, AtPUP5 overexpression increases RF accumulation following external application. In contrast, loss-of-function mutants do not display defects in bulk RF uptake at the whole-plant level, indicating that AtPUP5 is not essential for global riboflavin acquisition. Notably, AtPUP5 deficiency results in RF overaccumulation in reproductive organs, including inflorescences, siliques, and seeds, irrespective of external RF supply. This organ-specific phenotype is fully suppressed by genetic complementation and coincides spatially with strong AtPUP5 promoter activity in reproductive tissues. These findings suggest that AtPUP5 contributes to the regulation of localized riboflavin distribution in reproductive tissues. Together, our study provides a molecular framework for plasma membrane-mediated regulation of riboflavin distribution in plants and highlights spatial regulation as an important component of flavin homeostasis.
Reference Key
openalex_W7161133118 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Rui Shibata, Hikari Kuwata, T. Sugimoto, Madoka Kikuchi, Takahiro Ishikawa, Takahisa Ogawa
Journal plant and cell physiology
Year 2026
DOI
10.1093/pcp/pcag061
URL
https://doi.org/10.1093/pcp/pcag061
Keywords Keywords not found

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