Collection and direct amplification methods using the GlobalFiler™ kit for DNA recovered from common pipe bomb substrates.

Clicks: 159
ID: 30887
2019
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Abstract
When analyzing DNA from exploded pipe bombs, quantities are often in trace amounts, making DNA typing extremely difficult. Amplifying minute amounts of DNA can cause stochastic effects resulting in partial or uninterpretable profiles. Therefore, the initial DNA collection from "touch" evidence must be optimized to maximize the amount of DNA available for analysis. This proof-of-concept study evaluated two different swab types with two direct amplification strategies to identify the most effective method for recovering DNA from common pipe bomb substrates. PVC and steel pipes, electrical tape, and copper wire spiked with epithelial cells were swabbed with cotton or microFLOQ® Direct Swabs and amplified directly or via a pre-treatment prior to STR amplification. Not only was the microFLOQ® Direct Swab protocol the quickest method with the least risk of contamination, but in combination with direct amplification, the microFLOQ® Direct Swabs also generated the most complete STR profiles.
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tasker2019collectionscience Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Tasker, Esiri;Mayes, Carrie;LaRue, Bobby;Hughes-Stamm, Sheree;
Journal Science & justice : journal of the Forensic Science Society
Year 2019
DOI
S1355-0306(19)30029-2
URL
Keywords Keywords not found

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