Monodehydroascorbate Reductase in Spinach Chloroplasts and Its Participation in Regeneration of Ascorbate for Scavenging Hydrogen Peroxide

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ID: 307084
1984
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Abstract
The primary reaction product of chloroplast ascorbate peroxidase activity was shown to be monodehydroascorbate radical (MDA). MDA reductase (EC 1.6.5.4) was localized in spinach chloroplast stroma. The MDA reductase activity of spinach chloroplasts, using NAD(P)H as electron donor, could account for the regeneration of ascorbate from MDA produced by ascorbate peroxidase activity. In the absence of MDA reductase, MDA disproportionated to ascorbate (AsA) and dehydroascorbate (DHA). The DHA was reduced to AsA by DHA reductase (EC 1.8.5.1) in chloroplasts. Both NADH and NADPH served as the electron donor of partially purified MDA reductase from spinach leaves.
Reference Key
openalex_W1842909822 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors M. Anwar Hossain, Yoshiyuki Nakano, Kozi Asada
Journal plant and cell physiology
Year 1984
DOI
10.1093/oxfordjournals.pcp.a076726
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