SIZ1-Mediated Sumoylation of ICE1 ControlsCBF3/DREB1AExpression and Freezing Tolerance inArabidopsis
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ID: 306816
2007
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Abstract
Abstract SIZ1 is a SUMO E3 ligase that facilitates conjugation of SUMO to protein substrates. siz1-2 and siz1-3 T-DNA insertion alleles that caused freezing and chilling sensitivities were complemented genetically by expressing SIZ1, indicating that the SIZ1 is a controller of low temperature adaptation in plants. Cold-induced expression of CBF/DREB1, particularly of CBF3/DREB1A, and of the regulon genes was repressed by siz1. siz1 did not affect expression of ICE1, which encodes a MYC transcription factor that is a controller of CBF3/DREB1A. A K393R substitution in ICE1 [ICE1(K393R)] blocked SIZ1-mediated sumoylation in vitro and in protoplasts identifying the K393 residue as the principal site of SUMO conjugation. SIZ1-dependent sumoylation of ICE1 in protoplasts was moderately induced by cold. Sumoylation of recombinant ICE1 reduced polyubiquitination of the protein in vitro. ICE1(K393R) expression in wild-type plants repressed cold-induced CBF3/DREB1A expression and increased freezing sensitivity. Furthermore, expression of ICE1(K393R) induced transcript accumulation of MYB15, which encodes a MYB transcription factor that is a negative regulator of CBF/DREB1. SIZ1-dependent sumoylation of ICE1 may activate and/or stabilize the protein, facilitating expression of CBF3/DREB1A and repression of MYB15, leading to low temperature tolerance.
| Reference Key |
openalex_W1983870100
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| Authors | Kenji Miura, Jing Bo Jin, Ji‐Young Lee, Chan Yul Yoo, Vicki Stirm, Tomoko Miura, Edward N. Ashworth, Ray A. Bressan, Dae‐Jin Yun, Paul M. Hasegawa |
| Journal | The Plant cell |
| Year | 2007 |
| DOI |
10.1105/tpc.106.048397
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| URL | |
| Keywords | Keywords not found |
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