Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

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ID: 306708
2016
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Abstract
While transformation of the major monocot crops is currently possible, the process typically remains confined to one or two genotypes per species, often with poor agronomics, and efficiencies that place these methods beyond the reach of most academic laboratories. Here, we report a transformation approach involving overexpression of the maize (Zea mays) Baby boom (Bbm) and maize Wuschel2 (Wus2) genes, which produced high transformation frequencies in numerous previously nontransformable maize inbred lines. For example, the Pioneer inbred PHH5G is recalcitrant to biolistic and Agrobacterium tumefaciens transformation. However, when Bbm and Wus2 were expressed, transgenic calli were recovered from over 40% of the starting explants, with most producing healthy, fertile plants. Another limitation for many monocots is the intensive labor and greenhouse space required to supply immature embryos for transformation. This problem could be alleviated using alternative target tissues that could be supplied consistently with automated preparation. As a major step toward this objective, we transformed Bbm and Wus2 directly into either embryo slices from mature seed or leaf segments from seedlings in a variety of Pioneer inbred lines, routinely recovering healthy, fertile T0 plants. Finally, we demonstrated that the maize Bbm and Wus2 genes stimulate transformation in sorghum (Sorghum bicolor) immature embryos, sugarcane (Saccharum officinarum) callus, and indica rice (Oryza sativa ssp indica) callus.
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Authors Keith Lowe, Emily Wu, Ning Wang, George Hoerster, Craig Hastings, Myeong‐Je Cho, Chris Scelonge, Brian Lenderts, Mark A. Chamberlin, Josh Cushatt, Lijuan Wang, Larisa Ryan, Tanveer Khan, Julia Chow-Yiu, Wei Hua, Maryanne Yu, Jenny Banh, Zhongmeng Bao, Kent Brink, Elizabeth Igo, Bhojaraja Rudrappa, PM Shamseer, W.A. Bruce, Lisa J. Newman, Bo Shen, Peizhong Zheng, Dennis Bidney, Carl Falco, Jim Register, Zuo‐Yu Zhao, Deping Xu, Todd J. Jones, William Gordon‐Kamm
Journal The Plant cell
Year 2016
DOI
10.1105/tpc.16.00124
URL
https://doi.org/10.1105/tpc.16.00124
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