Hybridization of synthetic oligodeoxyribonucleotides to ΦX174 DNA: the effect of single base pair mismatch

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ID: 305970
1979
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Abstract
Oligodeoxyribonucleotides complementary to the DNA of the wild type (wt) bacteriophage Φ X 174 have been synthesized by the phosphotriester method. The ollgomers, 11, 14, and 17 bases long, are complementary to the region of the DNA which accounts for the am-3 point mutation. When hybridized to am-3 DNA, the oligonucleotides form duplexes with a single base pair mismatch. The thermal stability of the duplexes formed between wt and am-3 DNAs has been measured. The am-3 DNA: ollgomer duplexes dissociate at a temperature about 10°C lower than the corresponding wt DNA: ollgomer duplexes. This dramatic decrease in thermal stability due to a single mismatch makes it possible to eliminate the formation of the mismatched duplexes by the appropriate choice of hybridization temperature. These results are discussed with respect to the use of oligonucleotides as probes for the isolation of specific cloned DNA sequences.
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Authors R. Bruce Wallace, Jacquelin B. Shaffer, Robert F. Murphy, James Bonner, Tadaaki Hirose, Keiichi Itakura
Journal Nucleic Acids Research
Year 1979
DOI
10.1093/nar/6.11.3543
URL
https://doi.org/10.1093/nar/6.11.3543
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