DNA diversity among clinical isolates ofHelicobacter pyloridetected by PCR-based RAPD fingerprinting

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ID: 305659
1992
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Abstract
The RAPD (or AP-PCR) DNA fingerprinting method was used to distinguish among clinical isolates of Helicobacter pylori , a bacterium whose long term carriage is associated with gastritis, peptic ulcers and gastric carcinomas. This method uses arbitrarily chosen oligonucleotides to prime DNA synthesis from genomlc sites to which they are fortuitously matched, or almost matched. Most 10-nt primers with ss 60% G + C yielded strain-specific arrays of up to 15 prominent fragments, as did most longer (≥ 17-nt) primers, whereas most 10-nt primers with 50% G + C did not. Each of 64 Independent H.pylori isolates, 60 of which were from patients in the same hospital, was distinguishable with a single RAPD primer, which suggests a high level of DNA sequence diversity within this species. In contrast, isolates from initial and followup biopsies were indistinguishable in each of three cases tested.
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openalex_W2080490975 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Natalia Akopyanz, N O Bukanov, T. Ulf Westblom, Stephen Kresovich, Douglas E. Berg
Journal Nucleic Acids Research
Year 1992
DOI
10.1093/nar/20.19.5137
URL
https://doi.org/10.1093/nar/20.19.5137
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