PARP-1 and Ku compete for repair of DNA double strand breaks by distinct NHEJ pathways

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ID: 305148
2006
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Abstract
Poly(ADP-ribose)polymerase 1 (PARP-1) recognizes DNA strand interruptions in vivo and triggers its own modification as well as that of other proteins by the sequential addition of ADP-ribose to form polymers. This modification causes a release of PARP-1 from DNA ends and initiates a variety of responses including DNA repair. While PARP-1 has been firmly implicated in base excision and single strand break repair, its role in the repair of DNA double strand breaks (DSBs) remains unclear. Here, we show that PARP-1, probably together with DNA ligase III, operates in an alternative pathway of non-homologous end joining (NHEJ) that functions as backup to the classical pathway of NHEJ that utilizes DNA-PKcs, Ku, DNA ligase IV, XRCC4, XLF/Cernunnos and Artemis. PARP-1 binds to DNA ends in direct competition with Ku. However, in irradiated cells the higher affinity of Ku for DSBs and an excessive number of other forms of competing DNA lesions limit its contribution to DSB repair. When essential components of the classical pathway of NHEJ are absent, PARP-1 is recruited for DSB repair, particularly in the absence of Ku and non-DSB lesions. This form of DSB repair is sensitive to PARP-1 inhibitors. The results define the function of PARP-1 in DSB repair and characterize a candidate pathway responsible for joining errors causing genomic instability and cancer.
Reference Key
openalex_W2050164541 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Minli Wang, Wei‐Zhong Wu, Wenqi Wu, Bustanur Rosidi, Lihua Zhang, Huichen Wang, George Iliakis
Journal Nucleic Acids Research
Year 2006
DOI
10.1093/nar/gkl840
URL
https://doi.org/10.1093/nar/gkl840
Keywords Keywords not found

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