Enhancement of the Infectivity of Simian Virus 40 Deoxyribonucleic Acid With Diethylaminoethyl-Dextran2

Clicks: 2
ID: 304873
1968
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Star

Ranked #127 of 332 articles by views in JNCI Journal of the National Cancer Institute

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 332 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
The infectivity of viral RNA for mammalian cells in culture is greatly enhanced by diethylaminoethyl-dextran (DEAE-D). In this work a similar enhancing effect is shown for the infectious DNA of simian virus 40 (SV40). This phenomenon has enabled the development of a sensitive plaque method for the assay of infectious viral DNA; the reliability of the assay is in part accounted for by elimination of the need for exposure of the assay cells to hypertonic media. DEAE-D in Tris-buffered isosmolar media in concentrations of from 100 to 3000 μg/ml increases the infectivity of SV40 DNA up to 100,000 times. With DEAE-D's of molecular weight 26,000 to 2,000,000, DNA infectivity was directly related to molecular weight of DEAE-D. Paradoxically, DEAE-D produced a 20-fold reduction in infectivity of intact SV40. The degree of inhibition also depended on the molecular weight of DEAE-D, the number of SV40 plaques being inversely related to molecular weight. Since as much as 0.1% of viral infectivity can be recovered in the extracted DNA, it is now possible to prepare SV40 DNA with an infectivity titer high enough to initiate synchronous infection of all the cells in a culture.
Reference Key
openalex_W2292552118 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors James H. McCutchan, Joseph S. Pagano
Journal JNCI Journal of the National Cancer Institute
Year 1968
DOI
10.1093/jnci/41.2.351
URL
https://doi.org/10.1093/jnci/41.2.351
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.