Purification of Ascorbate Peroxidase in Spinach Chloroplasts; Its Inactivation in Ascorbate-Depleted Medium and Reactivation by Monodehydroascorbate Radical
Clicks: 1
ID: 304754
1987
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
0.0
/100
1 views
0 readers
AI Quality Assessment
Not analyzed
Readership in this journal
Ranked #69 of 94 articles by views in plant and cell physiology
Most read
Least read
Bar heights use a square-root scale.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Ascorbate specific peroxidase in chloroplasts was purified from spinach leaves. Spinach chloroplast peroxidase was a monomer with a molecular weight of about 30,000 and showed an absorption spectrum similar to a hemoprotein. The enzyme lost its activity within a minute in the absence of ascorbate under aerobic conditions. In addition to ascorbate, 20% sorbitol was necessary to stabilize the enzyme. The inactivation of the enzyme in the ascorbate-depleted medium was protected by other electron donors, pyrogallol, guaiacol and pyrocatechol, whose oxidation rates were very low compared with that of ascorbate. The inactivated enzyme recovered its activity with monodehydroascorbate radicals generated by the ascorbate-ascorbate oxidase system. A mechanism of inactivation and reactivation of ascorbate peroxidase is proposed.
| Reference Key |
openalex_W1872812400
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | Yoshiyuki Nakano, Kozi Asada |
| Journal | plant and cell physiology |
| Year | 1987 |
| DOI |
10.1093/oxfordjournals.pcp.a077268
|
| URL | |
| Keywords | Keywords not found |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.