Polymer support oligonucleotide synthesis XVIII1.2): use ofβ-cyanoethyi-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product

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ID: 302666
1984
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Abstract
Various 5′-0-N-protected deoxynucleoside-3′-0-β-cyanoethyl-N,N-dialkylamino-/N-morpholinophosphoramidites were prepared from β-cyanoethyl monochlorophosphoramidites of N,N-dimethylamine, N,N-diisopropylamine and N-morpholine. These active deoxynucleoside phosphites have successfully been used for oligodeoxy-nucleotide synthessis on controlled pore glass as polymer support and are very suitable for automated DNA-synthesis due to their stability in solution. The intermediate dichloro-β-cyanoethoxy-phosphine can easily be prepared free from any PCl3 contamination. The active monomers obtained from β-cyanoethyl monochloro N,N-diisopropylaminophosphoramidites are favoured. Cleavage of the oligonucleotide chain from the polymer support, N-deacylation and deprotection of β-cyanoethyl group from the phosphate triester moiety can be performed in one step with concentrated aqueous ammonia. Mixed oligodeoxynucleotides are characterized by the sequencing method of Maxam and Gilbert.
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openalex_W1984286262 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors N. D. SINHA, J. Biernat, J. F. A. McManus, H. Köster
Journal Nucleic Acids Research
Year 1984
DOI
10.1093/nar/12.11.4539
URL
https://doi.org/10.1093/nar/12.11.4539
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