PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification

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ID: 302149
2009
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Abstract
PrimerBank (http://pga.mgh.harvard.edu/primerbank/) is a public resource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and Quantitative PCR (QPCR). A total of 306 800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as Tm, location on the transcript and amplicon size. For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. There are several ways to search for primers for the gene(s) of interest, such as by: GenBank accession number, NCBI protein accession number, NCBI gene ID, PrimerBank ID, NCBI gene symbol or gene description (keyword). In all, 26 855 primer pairs covering most known mouse genes have been experimentally validated by QPCR, agarose gel analysis, sequencing and BLAST, and all validation data can be freely accessed from the PrimerBank web site.
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openalex_W2092288031 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Athanasia Spandidos, Xiaowei Wang, Huajun Wang, Brian Seed
Journal Nucleic Acids Research
Year 2009
DOI
10.1093/nar/gkp1005
URL
https://academic.oup.com/nar/article-pdf/38/suppl_1/D792/11217435/gkp1005.pdf
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