Transcriptome analysis by strand-specific sequencing of complementary DNA

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ID: 302140
2009
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Abstract
High-throughput complementary DNA sequencing (RNA-Seq) is a powerful tool for whole-transcriptome analysis, supplying information about a transcript's expression level and structure. However, it is difficult to determine the polarity of transcripts, and therefore identify which strand is transcribed. Here, we present a simple cDNA sequencing protocol that preserves information about a transcript's direction. Using Saccharomyces cerevisiae and mouse brain transcriptomes as models, we demonstrate that knowing the transcript's orientation allows more accurate determination of the structure and expression of genes. It also helps to identify new genes and enables studying promoter-associated and antisense transcription. The transcriptional landscapes we obtained are available online.
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openalex_W2097337885 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Dmitri Parkhomchuk, Tatiana Borodina, Vyacheslav Amstislavskiy, Maria Banaru, Linda Hallen, Sylvia Krobitsch, Hans Lehrach, А. В. Солдатов
Journal Nucleic Acids Research
Year 2009
DOI
10.1093/nar/gkp596
URL
https://academic.oup.com/nar/article-pdf/37/18/e123/16753757/gkp596.pdf
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