Diversity Arrays: a solid state technology for sequence information independent genotyping

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ID: 300616
2001
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Abstract
Here we present the successful application of the microarray technology platform to the analysis of DNA polymorphisms. Using the rice genome as a model, we demonstrate the potential of a high-throughput genome analysis method called Diversity Array Technology, DArT‘. In the format presented here the technology is assaying for the presence (or amount) of a specific DNA fragment in a representation derived from the total genomic DNA of an organism or a population of organisms. Two different approaches are presented: the first involves contrasting two representations on a single array while the second involves contrasting a representation with a reference DNA fragment common to all elements of the array. The Diversity Panels created using this method allow genetic fingerprinting of any organism or group of organisms belonging to the gene pool from which the panel was developed. Diversity Arrays enable rapid and economical application of a highly parallel, solid-state genotyping technology to any genome or complex genomic mixtures.
Reference Key
openalex_W2160759337 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Damian Jaccoud, Kun Peng, Douglas L. Feinstein, Andrzej Kilian
Journal Nucleic Acids Research
Year 2001
DOI
10.1093/nar/29.4.e25
URL
https://doi.org/10.1093/nar/29.4.e25
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